GENERAL |
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Purdue University Cytometry Labs
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ISAC |
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National Flow Cytometry Resource Center |
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BIOSAFETY |
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OSHA Homepage |
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Belgian Biosafety Server |
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ANTIBODIES |
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Biocompare |
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The Antibody Resource |
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The Salk Institute Commercial Links |
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CD Taxonomic Key Program |
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Mouse CDs, other antigens table |
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Human CDs |
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Antibody Cross-Reactivity Source |
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NIH Non-Human primate resoursce |
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PROTOCOLS |
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The Altman Lab |
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The Telford Lab |
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UPenn SDM Flow Cytometry |
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EBioscience Protocols |
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The Herzenberg Lab |
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The Nolan Lab |
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Jackson ImmunoResearch Technical Center |
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CONJUGATING FLUOROCHROMES |
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Molecular Probes Handbook |
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FITC, PE, Cy5PE, Cy7PE, TxRed, APC, Cy5, Cy7APC, Cascade Blue, Biotin (Mario Roederer) |
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Conjugation of monoclonal antibodies (Altman Lab) |
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Fluorochrome conjugation by amine modification (Telford Lab) |
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Hapten anti-Hapten labeling (Telford Lab) |
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A quick and simple method for making FITC or biotin conjugates (TIG) |
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Quantum Dots Conjugation (EviTag Technologies) |
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PE-conjugation protocol (Salk Institute) |
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Antibody Modification (Sigma) |
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Biotinylation of Antibodies (Mario Roederer) |
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SOFTWARE |
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FlowJo |
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FACSPress and FACSAssistant |
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DeNovoSoftware |
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WinMDI (free on Software for FlowCytometry page) |
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Verity Software House
(Video Tutorials for WinList, ModFit, QuantiCalc) |
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Convert FSC to ASCII |
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CytoWin (Free download, Daniel Valuot) |
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DaveyFCM.exe, free Program written to analyse Coulter Elite data (written at Aberystwith) |
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Scatgraph (for Scatron Argus 1.0; Davey and Kell) for MSDOS computers |
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Flow Cytometry Software Workshop (Geoffery Osborn, Uni of Queensland) |
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Cluster Analysis Software (located on Comprehensive Free Software List at Purdue)
AUTOKLUS |
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Cylchred Version 1.0.2 for Windows 95 (free cell cycle analysis, Cardiff) |
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FLUORESCENT PROTEINS |
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Fluorescent Proteins Colour
Palette ( Olympus) |
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Nanolight Technologies |
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Evrogen |
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Invitrogen Molecular Probes |
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TUTORIALS |
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FRET with Fluorescent proteins (Nikon) |
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Fundamentals of Image Processing |
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Digital Imaging |
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Basic tutorials about fluorescence |
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Compensation |
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Compensation (Mario Roederer) |
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Critical Aspects of Staining for Flow Cytometry (Alice Givan) |
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Vantage SE jet optimization |
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Sort recovery calculator |
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Median Fluorescence Intensity (Eric Martz) |
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BD Intro to flow Cytometry |
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BD Diva Software Overview |
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INTRACELLULAR FLOW CYTOMETRY |
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FOXP3-staining protocol |
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Maecker Lab Tools |
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Cytokine staining protocol (EBioscience) |
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Cytokine staining protocol (Herzenberg Lab) |
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Multi-colour intracellular staining for cytokines (TIG) |
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BCL2-staining protocol (Penn) |
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Permeabilizing Solutions (Penn) |
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Ki67 (BD Biosciences) |
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HSP70 expression and nuclear translocation (Telford Lab) |
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3-colour surface&intracellular labeling (UTMD-Science park) |
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2-colour cell Cycle with BRDU-PI protocol (UTMD-Science Park) |
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GFP and Cell Cycle Analysis ( Princeton Uni) |
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Cell Cycle Analysis with PI, Acridine Orange, DAPI ( Princeton Uni) |
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Cyclin D1 and D2/D3 (Telford) |
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INSTRUMENT MANUALS |
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BD FACScan™ Usage Instructions
(Uni of Chicago) |
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OTHER HELPFUL LINKS |
Transgenic and knockout mice ( UK) |
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ATCC |
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Immunohistochemistry (Millipore) |
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BD Fluorescence Spectrum viewer |
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Molecular probes Fluorescence Spectra Viewer |
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HistoSearch |
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IHC World |
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Qdots(pdf of Patricia Waley presentation) |
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