Research Flow & Imaging
Cytometry Resource


 
 
Resources
 

barteneva

 


GENERAL

 

 

Purdue University Cytometry Labs

 

 

ISAC

 

National Flow Cytometry Resource Center

 

BIOSAFETY

 

 

OSHA Homepage

 

Belgian Biosafety Server

 

ANTIBODIES

 

 

Biocompare

 

The Antibody Resource

 

The Salk Institute Commercial Links

 

CD Taxonomic Key Program

 

Mouse CDs, other antigens table

 

Human CDs

 

Antibody Cross-Reactivity Source

 

NIH Non-Human primate resoursce

 

 

 

PROTOCOLS

 

 

The Altman Lab

 

The Telford Lab

 

UPenn SDM Flow Cytometry

 

EBioscience Protocols

 

The Herzenberg Lab

 

The Nolan Lab

 

Jackson ImmunoResearch Technical Center

 

CONJUGATING FLUOROCHROMES

 

 

Molecular Probes Handbook

 

FITC, PE, Cy5PE, Cy7PE, TxRed, APC, Cy5, Cy7APC, Cascade Blue, Biotin (Mario Roederer)

 

Conjugation of monoclonal antibodies (Altman Lab)

 

Fluorochrome conjugation by amine modification (Telford Lab)

 

Hapten anti-Hapten labeling (Telford Lab)

 

A quick and simple method for making FITC or biotin conjugates (TIG)

 

Quantum Dots Conjugation (EviTag Technologies)

 

PE-conjugation protocol (Salk Institute)

 

Antibody Modification (Sigma)

 

Biotinylation of Antibodies (Mario Roederer)

 

SOFTWARE

 

 

FlowJo

 

FACSPress and FACSAssistant

 

DeNovoSoftware

 

WinMDI (free on Software for FlowCytometry page)

 

Verity Software House

(Video Tutorials for WinList, ModFit, QuantiCalc)

 

Convert FSC to ASCII

 

CytoWin (Free download, Daniel Valuot)

 

DaveyFCM.exe, free Program written to analyse Coulter Elite data (written at Aberystwith)

 

Scatgraph (for Scatron Argus 1.0; Davey and Kell) for MSDOS computers

 

Flow Cytometry Software Workshop (Geoffery Osborn, Uni of Queensland)

 

Cluster Analysis Software (located on Comprehensive Free Software List at Purdue)

AUTOKLUS

 

Cylchred Version 1.0.2 for Windows 95 (free cell cycle analysis, Cardiff)

 

FLUORESCENT PROTEINS

 

 

Fluorescent Proteins Colour

Palette ( Olympus)

 

Nanolight Technologies

 

Evrogen

 

Invitrogen Molecular Probes

 

TUTORIALS

 

 

FRET with Fluorescent proteins (Nikon)

 

Fundamentals of Image Processing

 

Digital Imaging

 

Basic tutorials about fluorescence

 

Compensation

 

Compensation (Mario Roederer)

 

Critical Aspects of Staining for Flow Cytometry (Alice Givan)

 

Vantage SE jet optimization

 

Sort recovery calculator

 

Median Fluorescence Intensity (Eric Martz)

 

BD Intro to flow Cytometry

 

BD Diva Software Overview

 

 

 

INTRACELLULAR FLOW CYTOMETRY

 

 

FOXP3-staining protocol

 

Maecker Lab Tools

 

Cytokine staining protocol (EBioscience)

 

Cytokine staining protocol (Herzenberg Lab)

 

Multi-colour intracellular staining for cytokines (TIG)

 

BCL2-staining protocol (Penn)

 

Permeabilizing Solutions (Penn)

 

Ki67 (BD Biosciences)

 

HSP70 expression and nuclear translocation (Telford Lab)

 

3-colour surface&intracellular labeling (UTMD-Science park)

 

2-colour cell Cycle with BRDU-PI protocol (UTMD-Science Park)

 

GFP and Cell Cycle Analysis ( Princeton Uni)

 

Cell Cycle Analysis with PI, Acridine Orange, DAPI ( Princeton Uni)

 

Cyclin D1 and D2/D3 (Telford)

 

INSTRUMENT MANUALS

 

 

BD FACScan™ Usage Instructions

(Uni of Chicago)

 

OTHER HELPFUL LINKS

Transgenic and knockout mice ( UK)

 

ATCC

 

Immunohistochemistry (Millipore)

 

BD Fluorescence Spectrum viewer

 

Molecular probes Fluorescence Spectra Viewer

 

HistoSearch

 

IHC World

 

Qdots(pdf of Patricia Waley presentation)

 

 

home / news

technology

research

forms

calendars

imaging cytometry

seminars

events

>resources<

FAQs

collaborators

contact

directions

back

home / news technology forms calendars imaging cytometry seminars events research >resources< FAQs collaborators contact directions back

 

 
© 2011 All rights reserved. Please contact webmaster with any technical problems, or the Director of Flow & Imaging Cytometry with any requests.
Immune Disease Institute (IDI) is an affiliate of Children's Hospital Boston and Harvard Medical School.